Mini Liposome Extruder

Convenient

• Prepare small or large, unilamellar vesicles (SUVs or GUVs) by extrusion in an easy, rapid, & efficient manner 

• Design allows for the rapid cleaning of all wetted parts, reducing down-time between production of  vesicles from batch to batch. 

• Generate uniform populations of unilamellar vesicles without the use of solvents or detergents 

• Vesicle size readily controlled by polycarbonate membrane selection

Flexible

• Optional heating block allows the extrusion of vesicles at elevated temperatures, which is critical for the successful production of vesicles from phospholipids with a phase transition temperature above room temperature 

• Generate unilamellar vesicles ranging from 30nm – 1µm when assembled with appropriate polycarbonate membrane 

• Useful for a wide variety of lipid compositions 

• Interchangeable with 250µL & 1000µL syringes

Affordable

• Available for a fraction of the cost of a larger extruder 

• All replacement parts and accessories are available 

• Complete set includes: 

Mini-extruder 

Heating block/holder 

2-Gas Tight Syringes (1000uL) 

2 O-rings 

100 polycarbonate membranes (0.1µm) 

100 Polyester filter supports

Reliable

The particle size distribution of unilamellar vesicles prepared by extrusion is a function of the number of passes through the extruder membrane. 

Hydrated lipid films will initially form large, multi-lamellar vesicles. After the initial pass through a membrane, the particle size distribution will tend towards bimodal. 

After sufficient passes through the membrane, a unimodal, normal distribution is obtained. A minimum of eleven passes through the extruder membrane is recommended for most lipids. 

Placing the heating block assembly on a hot plate achieves rapid temperature control for lipids that have transition temperatures above room temperature.