Mini微型挤出器

Convenient

• Prepare small or large, unilamellar vesicles (SUVs or GUVs) by extrusion in an easy, rapid, & efficient manner 

• Design allows for the rapid cleaning of all wetted parts, reducing down-time between production of  vesicles from batch to batch. 

• Generate uniform populations of unilamellar vesicles without the use of solvents or detergents 

• Vesicle size readily controlled by polycarbonate membrane selection

Flexible

• Optional heating block allows the extrusion of vesicles at elevated temperatures, which is critical for the successful production of vesicles from phospholipids with a phase transition temperature above room temperature 

• Generate unilamellar vesicles ranging from 30nm – 1µm when assembled with appropriate polycarbonate membrane 

• Useful for a wide variety of lipid compositions 

• Interchangeable with 250µL & 1000µL syringes

Affordable

• Available for a fraction of the cost of a larger extruder 

• All replacement parts and accessories are available 

• Complete set includes: 

Mini-extruder 

Heating block/holder 

2-Gas Tight Syringes (1000uL) 

2 O-rings 

100 polycarbonate membranes (0.1µm) 

100 Polyester filter supports

Reliable

The particle size distribution of unilamellar vesicles prepared by extrusion is a function of the number of passes through the extruder membrane. 

Hydrated lipid films will initially form large, multi-lamellar vesicles. After the initial pass through a membrane, the particle size distribution will tend towards bimodal. 

After sufficient passes through the membrane, a unimodal, normal distribution is obtained. A minimum of eleven passes through the extruder membrane is recommended for most lipids. 

Placing the heating block assembly on a hot plate achieves rapid temperature control for lipids that have transition temperatures above room temperature.

便捷性

• 可通过挤压法轻松、快速、高效制备小型或大型单层脂质体（SUVs或GUVs）

• 特殊结构设计可实现所有润湿部件的快速清洁，有效缩短批次生产间隔时间

• 无需有机溶剂或去污剂即可生成粒径均一的单层脂质体群体

• 通过选择不同规格的聚碳酸酯膜即可精确控制脂质体尺寸

灵活性

• 选配加热模块支持高温环境挤压操作，对相变温度高于室温的磷脂制备至关重要

• 搭配相应规格聚碳酸酯膜可生成30纳米至1微米的单层脂质体

• 适用于多种脂质成分组合

• 可兼容250微升与1000微升注射器替换使用

经济性

• 成本仅为大型挤压设备的小部分

• 所有替换部件与配件均可持续供应

• 标准套装包含：

迷你挤压器主机

加热模块/固定支架

2支气密注射器（1000微升）

2个O型密封圈

100张聚碳酸酯滤膜（0.1微米）

100张聚酯滤膜支撑垫

可靠性

通过挤压法制备的单层脂质体粒径分布与穿膜次数直接相关。

水合脂质膜初始会形成多层结构大脂质体，首次穿膜后粒径分布呈双峰趋势。

经过充分穿膜处理后，可获得单峰正态分布。建议多数脂质材料至少完成11次穿膜操作。

将加热模块组装体置于热台可实现快速温控，特别适用于相变温度高于室温的脂质材料。